|
| Status |
Public on Mar 01, 2022 |
| Title |
Hypoosmotic_t120_n4 |
| Sample type |
RNA |
| |
|
| Source name |
perfused rat liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
time point: t120
tissue: liver
strain: wistar
Sex: Male
buffer: hypoosmotic (225 mosm/L)
|
| Treatment protocol |
Rat liver perfusions were conducted as previously described by Sies et al. [https://doi.org/10.1016/s0076-6879(78)52005-3.]
|
| Growth protocol |
Male wistar rats were kept under normal husbandry conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and labeling were performed according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization and washing were carried out in a SciGene 777 Microarray Oven (50 rpm, 45 °C for 16 hours) and in an Affymetrix GeneChip 450 Washer.
|
| Scan protocol |
Microarrays scanning and acquisition of signal intensities were performed on the Affymetrix GeneChip Scanner GCS3000 System.
|
| Description |
Gene expression data from hypoosmotic perfused rat liver
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console 4.0 (TAC 4.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 05, 2021 |
| Last update date |
Mar 01, 2022 |
| Contact name |
David Schöler |
| Organization name |
University Hospital Düsseldorf
|
| Street address |
Moorenstr. 5
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE183460 |
Expression data from normo- and hypoosmotic perfused rat liver [120min] |
| GSE183462 |
Expression data from normo- and hypoosmotic perfused rat liver |
|
