|
| Status |
Public on Jan 20, 2011 |
| Title |
VARI092T |
| Sample type |
RNA |
| |
|
| Source name |
Homo Sapiens
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: clear cell
mutation: CAD
mutation: ERCC3
mutation: FGD5
mutation: NOTCH2
mutation: USP24
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
10 ug of Qiagen-purified total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
|
| Description |
Gene expression ratios were obtained from human kidney tumor cell lines.
|
| Data processing |
The data were analyzed with MBNI re-mapped CDF files, version 12.
|
| |
|
| Submission date |
Jun 14, 2010 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL10175 |
| Series (1) |
| GSE22316 |
PBRM1 Knockdown in RCC Cell Lines |
|
| Relations |
| Reanalysis of |
GSM444575 |
