|
| Status |
Public on Jun 29, 2011 |
| Title |
heads-dark_dark-CircadianTime44-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
heads-dark_dark-CircadianTime44
|
| Organism |
Anopheles gambiae |
| Characteristics |
tissue: head
timecollected: CT44
colloquialtimeofday: subjectiveLateNight
strain: Pimperena
gender: female
lighttreatment: constantDark
|
| Growth protocol |
Female mated, but not blood-fed Anopheles gambiae (Pimperena S form) mosquitoes were maintained at 80% humidity and 75°C in either constant dark conditions or 11 hours full light with a 1 hour dawn and one hour dusk transitions. Experiment began with females 7-9 days old. Zeitgeber Time (ZT) with ZT12 defined as time of lights OFF under the light:dark cycle, and ZT0 defined as end of the dawn transition. Circadian Time (CT) with CT0 defined as subjective dawn, inferred from ZT0 of the previous light:dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
20-30 mosquitoes were collected and pooled per timepoint. Heads (including mouth parts and antenae) were separated from bodies (including appendges), and RNA extracted using TRIzol reagent (2 rounds) (Invitrogen) and sodium acetate precipitation.
|
| Label |
Streptavidin Phycoerythrin (SAPE)
|
| Label protocol |
Sample labeled using the Affymetrix 3'IVT Express Kit per manufaturer's protocol.
|
| |
|
| Hybridization protocol |
Sample hybridized to a Plasmodium/Anopheles Affymetrix GeneChip GeneChip expression array (Affymetrix 900511, GEO GLP1321) per manufaturer's protocol.
|
| Scan protocol |
Sample scanned on an Affymetix GeneChip Scanner 7G running Command Console version 3.0 using manufactuer's protocols.
|
| Description |
Female Anopheles gambiae heads from mosquitoes reared under constant dark conditions. Collected at CT44, subjective late night (56 hours after lights off at the beginning of experiment)
|
| Data processing |
Expression values were normalized using the GC-RMA algorithm in GeneSpring GX11 (Agilent Technologies) against all other head or body samples.
|
| |
|
| Submission date |
Jun 25, 2010 |
| Last update date |
Jun 29, 2011 |
| Contact name |
Samuel S.C. Rund |
| E-mail(s) |
[email protected]
|
| Phone |
1-574-631-1869
|
| URL |
http://www.nd.edu/~srund
|
| Organization name |
University of Notre Dame
|
| Department |
Biological Science
|
| Lab |
Duffield
|
| Street address |
Galvin Life Science
|
| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE22585 |
Genome-wide profiling of diel and circadian gene expression of the malaria vector Anopheles gambiae |
|
