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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 21, 2022 |
| Title |
KOP5 |
| Sample type |
SRA |
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| Source name |
Lung
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Knock out
tissue: Lung
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from lung tissue was prepared using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
RNA samples sequenced on HiSeq2500 using Illumina TruSeq v4 chemistry
FasgtQC, trimming and alignment with STAR were conducted. Heseq were used for rawcounts calling. Deseq2 were used for downstream analysis and normalized raw counts with gene symbol name were created
Genome_build: mm10
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| Submission date |
Oct 25, 2021 |
| Last update date |
Jun 21, 2022 |
| Contact name |
Shioko Kimura |
| E-mail(s) |
[email protected]
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| Organization name |
NIH
|
| Department |
CCR
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| Lab |
Lab of metabolism
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| Street address |
9000 Rockville Pike
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20874 |
| Country |
USA |
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| Platform ID |
GPL17021 |
| Series (1) |
| GSE186515 |
Dysregulation of gene expression in SCGB3A2 Knock-out mouse |
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| Relations |
| BioSample |
SAMN22560864 |
| SRA |
SRX12764575 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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