|
| Status |
Public on Jul 17, 2010 |
| Title |
08SE1188 |
| Sample type |
RNA |
| |
|
| Source name |
MMP-8 KO arthritic mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ankle joint
genotype/variation: MMP-8 KO
strain: C57BL/6
|
| Treatment protocol |
Arthritic mice were intraperitoneally injected with 150 ul of K/BxN serum on days 0 and 2. Mice were killed for ankle RNA extraction on day 7 after serum injection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ankle joints were pulverized in presence of liquid nitrogen and total RNA was extracted with Rneasy mini kit and QIAshredder (Qiagen) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 300 ng of total RNA, using the WT cDNA synthesis and amplification kit (Affymetrix) following the GeneChip whole transcript (WT) sense target labelling assay manual of Affymetrix
|
| |
|
| Hybridization protocol |
Samples were hybridised to Affymetrix Mouse Gene 1.0 ST genechip using Affymetrix standard hybridization protocol (GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The chips were scanned using an Affymetrix 7G Genechip Scanner 3000 with GeneChip Command Console 1.1 software (Affymetrix)
|
| Description |
Arthritis induced by transfer of K/BxN serum
|
| Data processing |
Data were processing by RMA algorithm using Partek Genomic Suite
|
| |
|
| Submission date |
Jul 16, 2010 |
| Last update date |
Jul 19, 2010 |
| Contact name |
Carmen Conde |
| E-mail(s) |
[email protected]
|
| Phone |
34 981 950 903
|
| Organization name |
Hospital Clinico Universitario
|
| Lab |
Laboratorio de Investigacion nº 10
|
| Street address |
Choupana s/n
|
| City |
Santiago de Compostela |
| State/province |
A Coruña |
| ZIP/Postal code |
15706 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE22971 |
Expression data from MMP-8 wild type and KO mice with or without arthritis |
|
