|
| Status |
Public on Jul 30, 2010 |
| Title |
Liver-severe |
| Sample type |
RNA |
| |
|
| Source name |
Liver-severe
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed 129svj and C57 BL/6 background
tissue: spleen
disease status: severe hepatomegaly
|
| Growth protocol |
Livers and spleens were remove from euthanized mice and acutely frozen on dry ice and subsequently stored in -80C freever.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen lipid mini tissue kit was used for the Total RNA extraction
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). Transfer 200μl of hyb solution to each array, then tape holes and parafilm. Hybridize 16 hours at 45° at 60rpm. Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
disease liver severe stage
|
| Data processing |
Data were processed using Partek Genomic Suite software v7.3. GC-RMA normalization was applied to normalize gene level intensity.
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Jul 22, 2010 |
| Last update date |
Jul 29, 2010 |
| Contact name |
Pramod K Mistry |
| E-mail(s) |
[email protected]
|
| Phone |
203 785 4649
|
| Fax |
203 785 3365
|
| Organization name |
Yale University
|
| Department |
Pediatrics
|
| Lab |
LMP 4093
|
| Street address |
333 Cedar Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06562 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE23086 |
Beyond the Macrophage: System-wide Cellular and Molecular Dysregulation in GBA1 Deficient Mice Recapitulates Human Non-neuronopathic, Type 1 Gaucher Disease |
|
