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| Status |
Public on Aug 17, 2022 |
| Title |
normal control, N4 |
| Sample type |
SRA |
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| Source name |
human
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| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
disease state: control
age: 36
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard illumina protacal
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Data processing |
sequenced reads were trimmed for adptor sequence and masked for low-complexily sequence
mapped to human reference genome
Obtain mapped data (reads, etc.), for subsequent transcription assembly and expression count
The distribution of reads compared to the genome in different regions of the reference genome was statistically analyzed, and the target regions were divided into CDS (coding region) Intron (Intron) Intergenic region and UTR (5' and 3 untranslated region).
The FPKM value of each gene/transcript in the sample was calculated, and the value was taken as the expression amount of gene/transcript in the sample
Genome_build: GRCh38.p13
Supplementary_files_format_and_content: Gene/transcript COUNT expression matrix
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| Submission date |
Nov 16, 2021 |
| Last update date |
Aug 17, 2022 |
| Contact name |
Min Liu |
| E-mail(s) |
[email protected]
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| Organization name |
China-Japan Friendship Hospital
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| Street address |
No.2 Yinghua Dong Street, Hepingli, Chao Yang District
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
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| Platform ID |
GPL23227 |
| Series (1) |
| GSE188938 |
Transcriptome sequencing and ceRNA (lncRNA-miRNA-mRNA) network construction identified the key molecular markers and signaling pathways of chronic thromboembolic pulmonary hypertension |
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| Relations |
| BioSample |
SAMN23180854 |
| SRA |
SRX13147785 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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