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Sample GSM573998 Query DataSets for GSM573998
Status Public on Sep 01, 2010
Title AE15N
Sample type RNA
 
Source name adjacent normal tissue
Organism Homo sapiens
Characteristics tissue type: esophageal squamous cell carcinoma (ESCC)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified by standard Trizol method and followed with qualification check by Agilent 2100.
Label Biotin
Label protocol As per manufacturer (Affymetrix).
 
Hybridization protocol Total RNA was reverse transcripted to cDNA, reverse transcripted to labelled aRNA, purified, fragmented, and hybridized to each array according to the manufacturer's instructions.
Scan protocol The arrays were then washed using Affymetrix fluidics stations, and scanned using the Affymetrix GeneChip Scanner 3000.
Description frozen tissue extracted by Trizol
Data processing Microarray data were normalized using the R function rma() in the Bioconductor package affy
 
Submission date Aug 03, 2010
Last update date Aug 03, 2010
Contact name Howard Yang
E-mail(s) [email protected]
Phone 2402765257
Organization name NCI
Street address 9609 Medical Center Dr.
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
 
Platform ID GPL97
Series (1)
GSE23400 Global gene expression profiling and validation in esophageal squamous cell carcinoma (ESCC)

Data table header descriptions
ID_REF
VALUE RMA signal (log2 scale)

Data table
ID_REF VALUE
222385_x_at 8.8912
222386_s_at 9.752
222387_s_at 7.532
222388_s_at 10.1645
222389_s_at 8.9527
222390_at 9.8908
222391_at 9.3545
222392_x_at 12.6185
222393_s_at 8.1803
222394_at 8.6554
222395_s_at 9.4915
222396_at 8.9563
222397_at 7.0904
222398_s_at 9.2394
222399_s_at 10.0598
222400_s_at 10.3281
222401_s_at 10.3563
222402_at 5.3907
222403_at 8.1094
222404_x_at 9.1151

Total number of rows: 22477

Table truncated, full table size 378 Kbytes.




Supplementary file Size Download File type/resource
GSM573998.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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