|
| Status |
Public on Aug 12, 2010 |
| Title |
LSK SRF-null 1 |
| Sample type |
RNA |
| |
|
| Source name |
LSK cells SRF KO 1
|
| Organism |
Mus musculus |
| Characteristics |
cell type: sorted LSK cells
strain: C57BL/6
genotype/variation: SRF KO
|
| Treatment protocol |
FACS-sorted LSK (Lin-Sca1+c-Kit+) cells were isolated from pIpC-treated Srf flx/flx ;Mx1-Cre and Srf+/+;Mx1-Cre pooled animals
|
| Growth protocol |
Srf flx/flx mice were crossed with the polyinosine-polycytidine (pIpC)-inducible Mx1-Cre recombinase mouse line. Induction was performed on 6 weeks-old mice by three intra-peritoneal (IP) injections of 200 µg/dose pIpC (Invivogen) every 2 days and mice were analyzed 35 days after the last injection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were collected from invalidated or not 105 LSKs (n=3 per genotype) using the RNeasy micro kit (Qiagen) according to manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
30 ng of total RNA were amplified and labeled using the NuGen Ovation RNA Amplification System V2 followed by the FL-Ovation cDNA Biotin Module V2 according to manufacturer’s protocol. (NuGEN Technologies Inc.).
|
| |
|
| Hybridization protocol |
3,75 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip Affymetrix MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 7G Scanner
|
| Description |
Gene expression data from LSK cells SRF KO
|
| Data processing |
The data were analyzed with GeneChip Operating Software 1.4
|
| |
|
| Submission date |
Aug 10, 2010 |
| Last update date |
Aug 23, 2010 |
| Contact name |
Virginie PENARD |
| E-mail(s) |
[email protected]
|
| Phone |
33-1-42 11 42 33
|
| Fax |
33-1-42 11 52 40
|
| Organization name |
INSERM U985
|
| Department |
Institut Gustave Roussy
|
| Street address |
39 rue Camille Desmoulins
|
| City |
Villejuif |
| ZIP/Postal code |
94805 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23556 |
Mx-Cre mediated deletion of SRF in murine hematopoietic LSK (Lin-Sca1+c-Kit+) cells |
|
