|
| Status |
Public on Apr 19, 2022 |
| Title |
Control heart tissues at P17_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: heart
genotype: Znhit1 flox/flox
age: postnatal day 17
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse hearts (atrial tissues were removed) were snap-frozen and stored at -80℃ until use. Total RNA was isolated using TRIzol regents.
RNA-seq transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 1μg of total RNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to GRCm38 whole genome using STAR (V2.7.1a) with default parameters.
Fragments per kilobase per million reads (FPKM) were calculated using cuffnorm (v2.2.1) with default parameters.
Genome_build: GRCm38
Supplementary_files_format_and_content: *_RNA_seq_FPKM_matrix.txt: Tab-delimited text files include FPKM value matrix at P17 and P25.
|
| |
|
| Submission date |
Jan 20, 2022 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Yingchao Shi |
| E-mail(s) |
[email protected]
|
| Phone |
15261891962
|
| Organization name |
Nanjing University
|
| Street address |
22 Hankou Road
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210093 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE194115 |
RNA-Seq for control and Znhit1-deficient heart tissues from postnatal day 17 and 25 mice |
| GSE194164 |
Znhit1-deficiency caused sarcoplasmic reticulum originated vacuolar cardiomyopathy |
|
| Relations |
| BioSample |
SAMN25145151 |
| SRA |
SRX13849813 |
