|
| Status |
Public on May 19, 2011 |
| Title |
Rad21 esiRNA, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
R1/E ESC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: R1/E ESC
RNAi: Rad21
|
| Treatment protocol |
R1/E ES cells were reversely transfected in a 12well plate using 800ng esiRNA and 2ul Lipofectamine2000 (Invitrogen) and 80000cells/well. Procedure according to manufacture's protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit protocol including DNaseI digest
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on Mouse 430 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mouse ESC after 48h RNAi against Rad21
|
| Data processing |
The data were normalized with GC-RMA method using Partek Genomics Suite 6.4 (6.09.0129). Properties: quantile normalizytion, GCRMA background correction, median polish probe set summarization
|
| |
|
| Submission date |
Sep 01, 2010 |
| Last update date |
May 19, 2011 |
| Contact name |
Maciej Paszkowski-Rogacz |
| Organization name |
Technische Universität Dresden
|
| Department |
UCC / Medical Faculty and University Hospital Carl Gustav Carus
|
| Lab |
Medical Systems Biology (Prof. Buchholz)
|
| Street address |
Tatzberg 47/49
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE23923 |
Expression data from Rad21 knock-down in ES cells |
| GSE24030 |
The Cohesin Complex Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity |
|
