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Sample GSM597097 Query DataSets for GSM597097
Status Public on Feb 01, 2011
Title AKR/JTCE liver sample batch 2
Sample type RNA
 
Channel 1
Source name Pooled mouse tissue
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
Treatment protocol Fed male mice age 7-9 weeks were given 2100 mg/kg trichloroethylene in corn oil (10 ml/kg) by gavage or corn oil as vehicle
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from liver using Qiagen mini kit per manufacturer's instruction. Reference samples were pooled RNA from several organs from C57Bl/6J male mouse.
Label cy 3
Label protocol Low RNA Input Fluuorescent Linear Amp Kit (Agilent 5184-3253) per manufacturer's instruction.
 
Channel 2
Source name liver
Organism Mus musculus
Characteristics strain: AKR/J
treatment: TCE
gender: male
Treatment protocol Fed male mice age 7-9 weeks were given 2100 mg/kg trichloroethylene in corn oil (10 ml/kg) by gavage or corn oil as vehicle
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from liver using Qiagen mini kit per manufacturer's instruction. Reference samples were pooled RNA from several organs from C57Bl/6J male mouse.
Label Cy 5
Label protocol Low RNA Input Fluuorescent Linear Amp Kit (Agilent 5184-3253) per manufacturer's instruction.
 
 
Hybridization protocol Aglient Hybridization kit catalog # 5190-0404 was followed per manufacturer's instruction
Scan protocol Agilent scanner G2505A model
Description liver sample batch 2
Data processing Feature Extraction Agilent . Data was filtered by only accepting those genes with 70% or more values present.
sample batch normalization: Samples were run by batch with the same sample used for batch normalization by COMBAT.
 
Submission date Sep 22, 2010
Last update date Feb 01, 2011
Contact name Blair Urquhart Bradford
E-mail(s) [email protected]
Phone 919-966-6343
Organization name University of North Carolina
Department Environmental Sciences and Engineering
Lab Envrionmental Genomics
Street address 135 Dauer Dr. CB 7431
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
 
Platform ID GPL7202
Series (1)
GSE24278 The effect of trichloroethylene on gene expression in mouse liver

Data table header descriptions
ID_REF
VALUE Log base 2 of Lowess normalized (Cy5/Cy3) ratio representing test/reference

Data table
ID_REF VALUE
A_51_P100063 -1.784068976
A_51_P100099 -1.171198839
A_51_P100174 -2.459199566
A_51_P100181 -0.094386304
A_51_P100227 -0.201124925
A_51_P100246 -0.723246357
A_51_P100289 -1.085756328
A_51_P100298 -1.550134059
A_51_P100327 0.645917566
A_51_P100379 -0.811718078
A_51_P100470 0.189231282
A_51_P100505 -0.271419472
A_51_P100565 -0.307805558
A_51_P100573 0.090412776
A_51_P100625 2.396200224
A_51_P100787 -0.806698757
A_51_P100828 0.610838244
A_51_P100852 -0.414292845
A_51_P100856 1.831534687
A_51_P100866 -0.032445103

Total number of rows: 25617

Table truncated, full table size 633 Kbytes.




Supplementary file Size Download File type/resource
GSM597097_US14702406_251486812427_S01_GE2-v5_91_0806_1_4.txt.gz 14.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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