|
| Status |
Public on Jun 28, 2011 |
| Title |
M16_WT_0h, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen, wildtype, untreated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
phenotype: wildtype
tissue: spleen
treatment: none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Shh mutant phenotypes were manually identified as wild-type or mutants. cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99°C for 5 minutes, then 42°C for 5 minutes, and centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). Transfer 200μl of hyb solution to each array, then tape holes and parafilm. Hybridize 16 hours at 45° at 60rpm. Fluidics washing is FS450_0001.
|
| Scan protocol |
Affymetrix GeneChIP Scanner 3000 7G.
|
| Description |
M16
Wildtype, untreated.
|
| Data processing |
RMA normalization using Expression Console.
|
| |
|
| Submission date |
Sep 23, 2010 |
| Last update date |
Jun 28, 2011 |
| Contact name |
Olivia Prazeres da Costa |
| E-mail(s) |
[email protected]
|
| Phone |
00498941404149
|
| Organization name |
Technische Universität München
|
| Department |
Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstr. 30
|
| City |
München |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE24327 |
Lack of Toll-like Receptor Signaling Improves Host Defense in Severe Septic Peritonitis in Severe Septic Peritonitis |
|
