|
| Status |
Public on Nov 15, 2005 |
| Title |
Gastrin Knockout parietal cell replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrin Knockout fundus
|
| Organism |
Mus musculus |
| Characteristics |
Gastrin Knockout, 2 month old male mice parietal cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately10-14Gastrin Knockout mice were dissected and the fundus was used to isolate parietal cells and purified by flow cytometry. Total RNA was extracted from the purified parietal cells by Trizol followed by an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 1 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Gastrin Knockout purified (>90% pure) parietal cells
|
| Data processing |
Affymetrix Microarray Suite version 4.0
|
| |
|
| Submission date |
Jun 06, 2005 |
| Last update date |
Nov 15, 2005 |
| Contact name |
Linda Samuelson |
| E-mail(s) |
[email protected]
|
| Phone |
734-764-9448
|
| Fax |
734-936-8813
|
| Organization name |
University of Michigan
|
| Department |
Molecular and Integrative Physiology
|
| Street address |
MED SCI II, # 7761, 1150 W. MEDICAL CENTER DRIVE
|
| City |
ANN ARBOR |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE2772 |
Parietal cell gene expression in the presence and absence of gastrin |
|
