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Status
Public on Jun 15, 2005
Title
Fetal heart control 6
Sample type
RNA
Source name
Control fetal heart (vehicle treated with corn oil on gd14.5 via oral gavage, collected on gd17.5)
Organism
Mus musculus Characteristics
C57Bl6N Mouse
Extracted molecule
total RNA
Extraction protocol
Trizol reagent was used to isolate total RNA from whole d17.5 fetal mouse hearts.
Label
Labeling and probe preparation was performed exactly as recommended by Affymetrix.
Label protocol
Labeling and probe preparation was performed exactly as recommended by Affymetrix, using the Superscript Choice System for RT/PCR (Gibco/BRL Life Technologies) for cDNA synthesis and the RNA Transcript Labeling Kit (ENZO) for cRNA preparation.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
gd17.5 fetal mouse hearts
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Jun 14, 2005
Last update date
Jun 14, 2005
Contact name
Evan A. Thackaberry
E-mail(s)
[email protected] Phone
505 272 6535
Fax
505 272-6749
Organization name
University of New Mexico
Department
College of Pharmacy
Lab
M.K. Walker
Street address
2502 Marble N.E.
City
Albuquerque
State/province
NM
ZIP/Postal code
87131
Country
USA
Platform ID
GPL81 Series (1)
GSE2812 Fetal mouse heart, TCDD dose-response series
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 100001_at
21.1
A
0.901946
100002_at
106.4
A
0.378184
100003_at
63.3
A
0.162935
100004_at
317.9
P
0.000805
100005_at
560.3
P
0.001354
100006_at
147.8
P
0.000562
100007_at
707.7
P
0.001354
100009_r_at
42.8
A
0.127645
100010_at
157.5
A
0.175989
100011_at
170.4
P
0.001141
100012_at
264.8
A
0.204022
100013_at
405.6
P
0.035163
100014_at
181.5
P
0.000468
100015_at
237.7
P
0.002617
100016_at
159.1
P
0.002228
100017_at
92.3
A
0.066865
100018_at
171.2
A
0.189687
100019_at
1593.9
P
0.000468
100020_at
321
P
0.008689
100021_at
23.7
A
0.602006
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