|
| Status |
Public on Jun 15, 2005 |
| Title |
Fetal heart 3 ug/kg TCDD rep2 |
| Sample type |
RNA |
| |
|
| Source name |
T3 fetal heart (treated on gd14.5 via oral gavage, collected on gd17.5)
|
| Organism |
Mus musculus |
| Characteristics |
C57Bl6N Mouse
gd17.5 fetal heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent was used to isolate total RNA from whole d17.5 fetal mouse hearts.
|
| Label |
Labeling and probe preparation was performed exactly as recommended by Affymetrix.
|
| Label protocol |
Labeling and probe preparation was performed exactly as recommended by Affymetrix, using the Superscript Choice System for RT/PCR (Gibco/BRL Life Technologies) for cDNA synthesis and the RNA Transcript Labeling Kit (ENZO) for cRNA preparation.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
gd17.5 fetal mouse hearts
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jun 14, 2005 |
| Last update date |
Jun 14, 2005 |
| Contact name |
Evan A. Thackaberry |
| E-mail(s) |
[email protected]
|
| Phone |
505 272 6535
|
| Fax |
505 272-6749
|
| Organization name |
University of New Mexico
|
| Department |
College of Pharmacy
|
| Lab |
M.K. Walker
|
| Street address |
2502 Marble N.E.
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87131 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE2812 |
Fetal mouse heart, TCDD dose-response series |
|
