|
| Status |
Public on Mar 03, 2011 |
| Title |
MOSE Intermediate Cells, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse ovarian surface epithelial cells in culture
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse ovarian surface epithelial (MOSE) cell
malignant potential: intermediate
|
| Growth protocol |
MOSE cell lines were routinely maintained in DMEM high glucose medium supplemented with 4% fetal bovine serum, 100 mg/ml each of penicillin and streptomycin, 5 mg/ml insulin, 5 mg/ml transferrin, and 5 ng/ml sodium selenite.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples for early (passage 13, 14, and 15), intermediate (63, 71, and 73), and late (136, 142, and 143) passages were isolated using the RNeasy Kit according to the manufacturers instructions and treated with ribonuclease-free deoxyribonuclease I. RNA samples were assayed on the Agilent 2100 BioAnalyzer for qualitative assessment and quantification.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
|
| |
|
| Hybridization protocol |
The RNA samples were processed using Affymetrix's GeneChip WT cDNA Synthesis and Amplification Kit (900672). Hybridization was done using Affymetrix Hybridization, Wash, and Stain Kit (part # 900720). Arrays were hybed for 16 hr at 45C. Samples were stained and washed using the Genechip Fluidics 450 station
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 HR scanner.
|
| Description |
4_p2.63.CEL
Gene Expression of MOSE cells at different transformation stages
|
| Data processing |
We utilized MicroArray Suite 5.0 (Affymetrix, Santa Clara, CA) to process raw microarray data. Data values were globally scaled to an average target intensity to allow inter-GeneChip comparisons.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Mar 03, 2011 |
| Contact name |
Eva M Schmelz |
| E-mail(s) |
[email protected]
|
| Organization name |
Virginia Tech
|
| Department |
HNFE
|
| Lab |
Schmelz
|
| Street address |
Virginia Tech
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE24789 |
Expression data from mouse ovarian surface epithelium cells at different stages of malignancy |
|
