|
| Status |
Public on Oct 27, 2010 |
| Title |
iPS cell line O4-2 cultured in KSR |
| Sample type |
RNA |
| |
|
| Source name |
iPS cell line indcued by Oct4 cultured in iCD1
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblast cells infected by Oct4
cell line: O4-2
growth conditions: cultured in KSR
|
| Treatment protocol |
Cells are cultured in indicated medium
|
| Growth protocol |
Cultured in 37℃ with 5% CO2 in the media descripted in sample source name.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Whole Transcript (WT) Sense Target Labeling Assay Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip MoGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
expression data from iPS cells at D3 post-retroviral infection
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Partek software.
|
| |
|
| Submission date |
Oct 21, 2010 |
| Last update date |
Oct 26, 2010 |
| Contact name |
liu jing |
| E-mail(s) |
[email protected]
|
| Organization name |
GIBH
|
| Street address |
190 Kai Yuan Avenue
|
| City |
Guangzhou |
| State/province |
guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE24861 |
Expression profile of MEFs, iPS, and ES in different culture condition |
| GSE24930 |
MEFs, iPS, and ES expression profiles, and OKS mediated reprogramming regulated by Sox2 and Klf4 |
|
