|
| Status |
Public on Dec 01, 2010 |
| Title |
WD iPSC-1 |
| Sample type |
RNA |
| |
|
| Source name |
WD iPSC (passage 8)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Wilson’s disease iPSC
|
| Treatment protocol |
WD fibroblasts (passage 5), WD iPSCs (passage 8) and H9 (passage 40) were used for RNA extraction, spontaneously differentiated cells in WD iPSCs and H9 were removed manually before Trizol treatment.
|
| Growth protocol |
WD fibroblasts were maintained in DMEM plus 10% fetal bovine serum (FBS), WD iPSCs and H9 were maintained on Matrigel with mTESR1 culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected by Accutase treatment. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Whole Transcript (WT) Sense Target Labeling Assay Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HuGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
WD iPSC-1(KSR+VPA)
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using Partek software.
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
Jan 23, 2015 |
| Contact name |
shiqiang zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE24997 |
DNA microarrays of Wilson's disease patient-specific induced pluripotent stem cells |
|
