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Sample GSM61893 Query DataSets for GSM61893
Status Public on Aug 08, 2005
Title DCV_24h_rep_a
Sample type RNA
 
Source name entire body
Organism Drosophila melanogaster
Characteristics Strain: Oregon R
Biomaterial provider UPR9022 - CNRS
Extracted molecule total RNA
Extraction protocol Samples of 45 flies were ground to a fine powder were ground to a fine powder using matched glass pestle and grinder, followed by the immediate addition of 600 µl of RNeasy lysis buffer (QIAGEN, Courtaboeuf, France). After initial tissue disruption, total RNA was extracted using the RNeasy system, following the manufacturer’s instructions.
Label biotin
Label protocol For each experimental type, three biologically independent samples were used. Biotinylated RNA was produced and hybridized to Affymetrix Drosophila GeneChip microarrays (Part Number 510548; Affymetrix, Santa Clara, USA) according to the method published in Irving et al, 2001.
 
Description Analytic steps were performed using the programs within Affymetrix Microarray Suite 5.0 (Affymetrix) or Excel (Microsoft, Redmond, WA) with a combination of built in functions and custom formulae. The raw data were sorted using the Absent/Marginal/Present flags generated by the Microarray Suite functions. Whilst an Absent might indicate that there was no mRNA of particular type present in a sample, Marginal and Absent flags may indicate problems with the hybridisation, therefore only data points marked as present were retained.
Data processing The remaining data mass for each microarray was then normalised to itself, making the median of all the present measurements one. The subsequent analysis of results was carried out on the genes present in all three replicates of a sample type.
 
Submission date Jun 20, 2005
Last update date Aug 08, 2005
Contact name Jean-Luc Imler
E-mail(s) [email protected]
Organization name CNRS
Department IBMC
Lab UPR9022
Street address 2, Allée Konrad Roentgen
City Strasbourg Cedex
ZIP/Postal code 67084
Country France
 
Platform ID GPL72
Series (1)
GSE2828 The Jak-STAT signaling pathway is required but not sufficient for the antiviral response of drosophila

Data table header descriptions
ID_REF
VALUE DCV injection at 24h, replicate a, normalized value
DETECTION P-VALUE DCV injection at 24h, replicate a, p-value
ABS_CALL DCV injection at 24h, replicate a, flag

Data table
ID_REF VALUE DETECTION P-VALUE ABS_CALL
141200_at 0.10601719 0.66268104 A
141201_at 0.047994267 0.64495987 A
141202_at 0.91332376 0.96388555 P
141203_at 0.60171914 0.83749825 P
141204_at 0.5 0.79846084 A
141205_at 0.18767908 0.6886335 A
141206_at 1.3424067 0.8595061 P
141207_at 0.2722063 0.71673554 P
141208_at 1.1762177 0.9268225 P
141209_at 0.4520057 0.78055364 P
141210_at 0.2256447 0.70110077 P
141211_at 0.14899713 0.6761929 A
141212_at 4.2729225 0.2777917 P
141213_at 0.13180515 0.6707488 P
141214_at 1.4806589 0.8057734 P
141215_at 1.2578796 0.8934431 P
141216_at 0.46131805 0.784001 A
141217_at 1.5007162 0.79819113 P
141218_at 1.0007162 0.99970126 P
141219_at 0.4319484 0.7731741 A

Total number of rows: 14010

Table truncated, full table size 453 Kbytes.




Supplementary file Size Download File type/resource
GSM61893.CEL.gz 2.6 Mb (ftp)(http) CEL
GSM61893.EXP.gz 484 b (ftp)(http) EXP

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