|
| Status |
Public on Jan 01, 2011 |
| Title |
cell25_stimulated_90min_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
human lymphoblastoid cell line 2525 BCR stimulated for 90 min
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary human b cells infected with EBV mutant 2525
genotype/variation: wtLMP2A knockout
|
| Treatment protocol |
Cells were seeded at 10^7 per 3ml and left untreated or stimulated for 90 min; BCR stimulation: 25ug/ml goat-anti-human IgG/M (dianova); LMP2A:mCD69 stimulation: 1ug/ml armenian hamster-anti-murineCD69 + 5ug/ml goat-anti-armenian hamster IgG (dianova)
|
| Growth protocol |
LCLs 2525 and 3696.10 were cultivated in RPMI-1640 media supplemented with 10% fetal bovine serum, penicillin (100U/ml), streptomycin (100μg/ml), sodium selenit (100nM), sodium pyruvate (1mM), bathocuproinedisulfonic acid (40nM) and 0.87‰ α-thioglycerol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted, using the RNeasy mini Kit (Qiagen), followed by DNase digest for 90min at 37°C; mRNA was transcribed into cDNA by NimbleGen
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen as described in 'NimbleChip Arrays Uer's Guide: Gene Expression Analysis v2.0'. See www.nimblegen.com
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen as described in 'NimbleChip Arrays Uer's Guide: Gene Expression Analysis v2.0'. See www.nimblegen.com
|
| Scan protocol |
Scanning was performed by NimbleGen as described in 'NimbleChip Arrays Uer's Guide: Gene Expression Analysis v2.0'. See www.nimblegen.com
|
| Description |
This sample is of the lymphoblastoid cell line 2525. It is the third of three technical replicates used in this experiment, each from separate cultures.
|
| Data processing |
The raw .call data was subjected to Multi-Lowess normalization.
|
| |
|
| Submission date |
Nov 17, 2010 |
| Last update date |
Jan 01, 2011 |
| Contact name |
Wolfgang Hammerschmidt |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Zentrum München
|
| Department |
Gene Vectors
|
| Street address |
Marchioninistr. 25
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11219 |
| Series (1) |
| GSE25434 |
Comparative gene expression analysis upon stimulation of the human BCR or an inducible LMP2A in EBV immortalized lymphoblastoid cell lines |
|
