Male Fischer 344 rats were administered Ochratoxin A (OTA) through the diet over two years. The initial daily dietary intake was 300 mg OTA/kg bw, but was held at 100 mg/rat after animal reached 333 g.
Extracted molecule
total RNA
Extraction protocol
Clonetech
Label
biotin
Label protocol
5 ug of cRNA was used to make biotinylated RNA
Hybridization protocol
standard Affymetrix procedure
Scan protocol
standard Affymetrix procedure
Description
T21_7 OTA kidney 21 days
Data processing
Affymetrix Microarray Suite version 5.0 followed by GEA