|
| Status |
Public on Nov 22, 2012 |
| Title |
KO replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow derived granulocyte monocyte precursors (GMPs) from Gfi1 knock-out mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Gfi1 knock-out mice
|
| Growth protocol |
All mice were housed under SPF conditions and all animal experiments were approved by the institutional animal ethics committee
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was purified using the Qiagen (Hilden, Germany) Rneasy Micro Kit according to the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
for array hybridization total RNA was amplified and labeled using the following kits from Nugen (San Carlos, California; USA): WT-Ovation Pico Amplification system, WT-Ovation Exon module and FL-Ovation cDNA Biotin Module V2 and the Affymetrix's Hybridization Wash and Stain kit
|
| |
|
| Hybridization protocol |
hybridization was done following the manufacturers instructions
|
| Scan protocol |
scanning was done on a Genechip scanner 3000 using the manufacturers instructions
|
| Description |
KO2
|
| Data processing |
data was processed using the AltAnalyze software (http://altanalyze.org) using standard RMA analysis settings
|
| |
|
| Submission date |
Nov 23, 2010 |
| Last update date |
Nov 22, 2012 |
| Contact name |
Cyrus Khandanpour |
| E-mail(s) |
[email protected]
|
| Phone |
+49 20172385212
|
| Organization name |
University Hospital Essen
|
| Department |
Hematology
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE25551 |
Expression data from bone marrow derived granulocyte monocyte precursors (GMPs) from wt -, Gfi1 knock-out -, human-Gfi1-SNP36N knock in and human wt-Gfi1 knock in mice |
|
