|
| Status |
Public on Jul 05, 2006 |
| Title |
left ventricular- 10 months old-lean 1 |
| Sample type |
RNA |
| |
|
| Source name |
10 months old SHHF lean rat, left ventricular
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: SHHF, Gender: Male,
Age: 10 months, Tissue: left ventricular, Other: hypertension, dilated cardiomyopathy starting from 14 months
|
| Biomaterial provider |
Charles River laboratory
|
| Treatment protocol |
None
|
| Growth protocol |
Animals were lodged in the Zootechnic service of the Louis Bugnard Institute. A veterinary oversees animal health and management. Temperature : 22 +/- 1 degrees. Cycles nights/days : 12 hours. Food : Teklad Global 16% Protein Rodent Diet (Harlan)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
General anesthesia by Isoflurane 4%. Heart extraction. Isolation of total RNA from left ventricular by : TRIzol (Invitrogen), homogeneisor Ika Ultra-Thurax T 25 (Janke and Kunkel), DNAfree (Ambion). Quality and quantity were determined by capillary electrophoresis (Agilent).
|
| Label |
33P
|
| Label protocol |
Reverse Transcription of 3 µg total RNA from each left ventricular with Superscript enzyme (Invitrogen).
Purification of cDNA labelled with 33P with NucleoSpin Extract (Macherey-Nagel)
|
| |
|
| Hybridization protocol |
Using one nylon membrane per animal for the hybridization (kit Array-Advantage GF, Ambion)
|
| Scan protocol |
Exposition of each membrane during 24 hours (Storage Phosphor Screen, Molecular Dynamics). Revelation with Scan Typhoon 9400 (resolution at 50 µm per pixel). Analysis of images by Image Quant 5.1
|
| Description |
Lean rats can be homozygous or heterozygous for the leptin receptor gene (+/+ or cp/+, cp means corpulent allele and is a recessive autosomic one, + means wild type)
|
| Data processing |
background subtraction, log transformation, normalization by total mean, exponential (back from log), scaling by a factor 10000.
|
| |
|
| Submission date |
Jul 05, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Philippe V Rouet |
| E-mail(s) |
[email protected]
|
| Phone |
00 33 5 31 22 41 19
|
| Organization name |
INSERM U1048
|
| Department |
Cardiology
|
| Lab |
Team 7
|
| Street address |
1 Ave Jean Poulhes
|
| City |
Toulouse |
| State/province |
BP84225 |
| ZIP/Postal code |
31432 |
| Country |
France |
| |
|
| Platform ID |
GPL2580 |
| Series (1) |
| GSE2876 |
Obesity impact on the left ventricular transcriptome of 4 and 10 months old SHHF rats |
|
