|
| Status |
Public on Dec 15, 2005 |
| Title |
YFPH-SomatosensoryCortex-replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
somatosensory cortex
|
| Organism |
Mus musculus |
| Characteristics |
Strain: Mixed YFPH (Ref. PubmedID:11086982) & C57BL/6j
Gender: male
Age: P86
Tissue: Somatosensory Cortex
Cell: YFP labeled layer 5 pyramidal neurons.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Somatosensory cortex was microdissected from 400 micron thick slice. The microdissected tissue chunks were dissociated by pronase E and trituration. Fluorescently labeled neurons from the dissociated cells were hand-sorted under a fluorescent dissection microscope using glass pipette with 50 micron diameter and lysed in XB buffer (Arcturus). Total RNA was extracted from the lysate using PicoPure Kit (Arcturus). (Detailed protocol is available at http://mouse.bio.brandeis.edu/protocol.pdf .)
|
| Label |
biotin
|
| Label protocol |
Total RNA from 34 cells was amplified and processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Layer 5 pyramidal neurons in somatosensory cortex.
|
| Data processing |
dCHIP PM-only method
|
| |
|
| Submission date |
Jul 05, 2005 |
| Last update date |
Dec 01, 2005 |
| Contact name |
Ken Sugino |
| Organization name |
Janelia Research Campus
|
| Lab |
NeuroSeq
|
| Street address |
19700 Helix Dr
|
| City |
Ashburn |
| State/province |
VA |
| ZIP/Postal code |
20147 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE2882 |
Cell type specific expression profiles of mouse forebrain neurons |
|
