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Status
Public on Jul 08, 2005
Title
Day6 Estrogen treatment replicate 1
Sample type
RNA
Source name
Thymuse
Organism
Mus musculus Characteristics
Femal C57BL/6, ovariectomized
Extracted molecule
total RNA
Label
biotin
Description
21-day-old felmale C57BL/6 mice from Harlan (Indianapolis, IN) were ovariectomized on day 26 and fed with phytoestrogen-free diet two days later (AIN-93G purified rodent diet, Dyets, In., Bethlehem, PA). Experimental treatments began on day 30. Animals received daily subcutaneous injections of 125 ng of E2 (Signma-Aldrich, St. Louis, MO) in 0.02 ml corn oil. RNA were extracted at day 6 after experiment started. Total 6 animals were treated. RNA samples were pooled from 3 animals, resulting two independent biological replicates.
Data processing
Affymetrix Microarray Suite Version 5.0
Submission date
Jul 07, 2005
Last update date
Jul 07, 2005
Contact name
Lei Liu
E-mail(s)
[email protected] Phone
217-265-5061
Organization name
University of Illinois at Urbana-Champaign
Department
W. M. Keck Center
Street address
1201 W. Gregory
City
Urbana
State/province
IL
ZIP/Postal code
61801
Country
USA
Platform ID
GPL339 Series (1)
GSE2889 Gene Expression Profiling of 17 beta-Estradiol and Genistein Effects on Mouse Thymus
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript wa present (P), absent (A), marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
56.7
P
0.013811
AFFX-BioB-M_at
75.3
P
0.00034
AFFX-BioB-3_at
48.4
P
0.001102
AFFX-BioC-5_at
142.4
P
0.00034
AFFX-BioC-3_at
80.4
P
0.000195
AFFX-BioDn-5_at
128.7
P
0.00007
AFFX-BioDn-3_at
874.1
P
0.000169
AFFX-CreX-5_at
1481
P
0.000044
AFFX-CreX-3_at
2462.8
P
0.000044
AFFX-DapX-5_at
11.6
M
0.050229
AFFX-DapX-M_at
13.1
A
0.227636
AFFX-DapX-3_at
1
A
0.988616
AFFX-LysX-5_at
1.4
A
0.804734
AFFX-LysX-M_at
8.3
A
0.51489
AFFX-LysX-3_at
17.5
P
0.006187
AFFX-PheX-5_at
1.3
A
0.891021
AFFX-PheX-M_at
8.5
A
0.672921
AFFX-PheX-3_at
5.7
A
0.574038
AFFX-ThrX-5_at
2.4
A
0.824672
AFFX-ThrX-M_at
7.6
A
0.425962
22690 610 Kbytes .Supplementary data files not provided
