|
| Status |
Public on Jul 20, 2022 |
| Title |
Icariin-treated BMSC |
| Sample type |
RNA |
| |
|
| Source name |
Icariin-treated BMSC
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: BMSC
gender: female
treatment: Icariin-treated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The integrity of RNA can be assessed by electrophoresis on a denaturing agarose gel. Intact total RNA run on a denaturing gel will have sharp 28S and 18S rRNA bands (eukaryotic samples). The 28S rRNA band should be approximately twice as intense as the 18S rRNA band. This 2:1 intensity ratio indicates that the RNA is intact.
|
| Label |
Cy3
|
| Label protocol |
NimbleGen One-Color DNA Labeling Kit
|
| |
|
| Hybridization protocol |
NimbleGen Hybridization System
|
| Scan protocol |
Axon GenePix 4000B scanner
|
| Description |
The rat BMSC which Icariin-treated
|
| Data processing |
NimbleScan v2.5 software
Raw data normalization and low intensity filtering: Raw signal intensities were normalized in RMA method by NimbleScan v2.5, and low intensity genes were filtered (Genes that Intensity >= 100.0 in that at least 1 out of 2 samples were chosen for further analysis). Quality assessment of gene data after filtering: Contains Box Plot and Scatter Plot for genes after filtering.
|
| |
|
| Submission date |
Jul 19, 2022 |
| Last update date |
Jul 20, 2022 |
| Contact name |
Hongmeng Yu |
| E-mail(s) |
[email protected]
|
| Organization name |
Eye & ENT Hospital, Fudan University
|
| Department |
Department of Otolaryngology
|
| Street address |
No.83, Fenyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platform ID |
GPL19519 |
| Series (1) |
| GSE208583 |
NimbleGen Rat Gene Expression Microarrays for 2 samples (icariin/DMSO-treated BMSC) |
|
