|
| Status |
Public on Jun 01, 2011 |
| Title |
MCF7_+_Dox_4hr_E2_2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7-tet-on-shCARM1
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Dox + E2
treatment time: 4 h
|
| Treatment protocol |
When the cells were treated with Dox, they were treated with 1ug/ml Dox for 5 days to induce knockingdown CARM1. The medium were changed to phenol-red free DMEM+charcoal stripped FBS for 3 days before treating with E2 (10nM)
|
| Growth protocol |
MCF7-tet-on-CARM1shRNA cells were grown in DMEM in 10% FBS. Three days before E2 treatment, the cells were switched to phenol-red free DMEM with 5% 6X charcoal stripped FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA were extracted from cells using Qiagen Rneasy Kit
|
| Label |
Cy3
|
| Label protocol |
Total mRNA was labeled using Agilent’s Quick Amp Labeling Kit.
|
| |
|
| Hybridization protocol |
Hybridization was perform at 65C for 17 hours according to Agilent Tecan HS Pro Hybridization Protocol
|
| Scan protocol |
The scan setting is 5um resolution, sigle pass using GenePix 4000A scanner. The data was extracted using Agilent Feature Extraction Software.
|
| Description |
Dox pre-treat, E2 4h
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Dec 21, 2010 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Wei Xu |
| Organization name |
UW-Madison
|
| Street address |
1400 University Ave
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE26259 |
Gene expression profiles of MCF7 with CARM1 knocked down |
|
