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Sample GSM645006 Query DataSets for GSM645006
Status Public on Jun 01, 2011
Title MCF7_+_Dox_0hr_E2_1
Sample type RNA
 
Source name MCF7-tet-on-shCARM1
Organism Homo sapiens
Characteristics treatment: Dox
treatment time: 4 h
Treatment protocol When the cells were treated with Dox, they were treated with 1ug/ml Dox for 5 days to induce knockingdown CARM1. The medium were changed to phenol-red free DMEM+charcoal stripped FBS for 3 days before treating with E2 (10nM)
Growth protocol MCF7-tet-on-CARM1shRNA cells were grown in DMEM in 10% FBS. Three days before E2 treatment, the cells were switched to phenol-red free DMEM with 5% 6X charcoal stripped FBS.
Extracted molecule total RNA
Extraction protocol Total mRNA were extracted from cells using Qiagen Rneasy Kit
Label Cy3
Label protocol Total mRNA was labeled using Agilent’s Quick Amp Labeling Kit.
 
Hybridization protocol Hybridization was perform at 65C for 17 hours according to Agilent Tecan HS Pro Hybridization Protocol
Scan protocol The scan setting is 5um resolution, sigle pass using GenePix 4000A scanner. The data was extracted using Agilent Feature Extraction Software.
Description Dox treat, no E2 treat
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Dec 21, 2010
Last update date Jun 01, 2011
Contact name Wei Xu
Organization name UW-Madison
Street address 1400 University Ave
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platform ID GPL4133
Series (1)
GSE26259 Gene expression profiles of MCF7 with CARM1 knocked down

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity

Data table
ID_REF VALUE
12 6.117178546
13 5.396451603
14 7.022866499
15 4.952758155
16 11.27744067
17 4.961969067
18 6.335299139
19 12.68918596
20 5.60442313
21 4.942106121
22 12.31521098
23 4.920742448
24 10.08784361
25 8.248721448
26 5.19191682
27 5.740750777
28 4.840825064
29 4.927527781
30 9.389303942
31 5.009006628

Total number of rows: 43376

Table truncated, full table size 747 Kbytes.




Supplementary file Size Download File type/resource
GSM645006.txt.gz 8.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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