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Status |
Public on Jun 01, 2011 |
Title |
MCF7-CARM1#13-DOX+E2-a |
Sample type |
RNA |
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Source name |
breast cancer cell line
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Organism |
Homo sapiens |
Characteristics |
cell type: breast cancer cell line cell line: MCF7-tet-on-CARM1 clone 13 treatment: Dox and D2
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Treatment protocol |
When the cells were treated with Dox, they were treated with 1ug/ml Dox for 4 days to induce CARM1 expression. The medium were changed to phenol-red free DMEM+charcoal stripped FBS for 2 days before treating with E2 (10nM)
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Growth protocol |
MCF7-tet-on-CARM1 cells were grown in DMEM in 10% FBS. Two days before E2 treatment, the cells were switched to phenol-red free DMEM with 5% 6X charcoal stripped FBS.
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Extracted molecule |
total RNA |
Extraction protocol |
mRNA were extracted from cells using Qiagen Rneasy Kit
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Label |
Cy3
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Label protocol |
Invitrogen SuperScript Double-Stranded cDNA Synthesis Kit was used to synthesize double-stranded cDNA that was going to be labeled using a NimbleGen One-Color DNA Labeling Kit.
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Hybridization protocol |
Hybridization was performed at 42C for 20hrs, according to NimbleGen gene expression arrays user’s guide (2010).
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Scan protocol |
One-color NimbleGen arrays were scanned with the MS 200 Microarray Scanner and the data were extract using the MS 200 Data Collection Software.
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Description |
Dox and E2 SOM01VSC Sample barcode: 5951702
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
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Submission date |
Jan 05, 2011 |
Last update date |
Jun 01, 2011 |
Contact name |
Wei Xu |
Organization name |
UW-Madison
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Street address |
1400 University Ave
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City |
Madison |
State/province |
WI |
ZIP/Postal code |
53706 |
Country |
USA |
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Platform ID |
GPL11219 |
Series (1) |
GSE26454 |
Gene expression profiles of CARM1 overexpression in MCF7 |
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