|
| Status |
Public on Jan 11, 2011 |
| Title |
PD-1 low CD8+ CD3+ T cells - rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood cells healthy adults
|
| Organism |
Homo sapiens |
| Characteristics |
cell phenotype: PD-1 low CD8+CD3+ Naïve T cells
sorting strategy: FACS
source: Peripheral blood cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using Trizol® (Invitrogen, Life Technologies Corporation). All RNA samples were checked for purity using a ND-1000 spectrophotometer (NanoDrop Technologies) and for integrity by electrophoresis on a 2100 BioAnalyzer (Agilent Technologies).
|
| Label |
Biotin
|
| Label protocol |
cDNA was synthesized using the SuperScript Choice cDNA synthesis kit (Invitrogen) and an oligo (dT) primer containing a T7 promoter. The T7 MEGAscript kit (Ambion) was used to amplify cRNA from the cDNA. The cRNA was reverse transcribed with biotinylated nucleotides using the Enzo BioArray High Yield RNA transcript labeling kit in the second round of cRNA synthesis, fragmented for hybridization.
|
| |
|
| Hybridization protocol |
Labeled targets were hybridized to Affymetrix Human genome U133 Plus 2.0 arrays on the Affymetrix GeneChip Array Station (GCAS) automation platform
|
| Scan protocol |
Scanning was performed on an Affymetrix GeneChip HT plate scanner, and Affymetrix software was used to perform image analysis and generate raw intensity data.
|
| Data processing |
Gene expression data was summarized with RMA.
|
| |
|
| Submission date |
Jan 07, 2011 |
| Last update date |
Jan 11, 2011 |
| Contact name |
Jaikumar Duraiswamy |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory Univesity
|
| Department |
Emory Vaccine Center and Department of Microbiology & Immunology
|
| Lab |
Ahmed lab
|
| Street address |
1510, Clifton Road, G211, Rollins Research Center
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26495 |
Phenotype, Function and Gene Expression Profiles of PD-1 high CD8 T cells in Healthy Human Adults |
|
