NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM65322 Query DataSets for GSM65322
Status Public on Feb 16, 2006
Title KIT_313A87
Sample type RNA
 
Source name breast
Organism Homo sapiens
Characteristics sample name: KIT_313A87
grade: 3
ggi: 1.61792
Extracted molecule total RNA
Extraction protocol Isolation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturer's instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)olation of RNA was performed using the Trizol method (Invitrogen) according to the manufacturer's instructions and was purified using RNeasy mini-columns (Qiagen, Valencia, CA). The quality of the RNA obtained from each tumour sample was assessed based on the RNA profile generated by the bioanalyzer (Agilent Inc)
Label biotin
Label protocol Approximately 1 microgram of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Tumor samples from Tamoxifen-only treated primary breast cancer patients
Data processing RMA Bioconductor
 
Submission date Jul 25, 2005
Last update date Sep 06, 2013
Contact name Benjamin Haibe-Kains
E-mail(s) [email protected]
Phone +14165818626
Organization name Princess Margaret Cancer Centre
Department Princess Margaret Research
Lab Bioinformatics and Computational Genomics
Street address 610 University Avenue
City Toronto
State/province Ontario
ZIP/Postal code M5G 2M9
Country Canada
 
Platform ID GPL96
Series (2)
GSE2990 Gene Expression Profiling in Breast Cancer: Understanding the Molecular Basis of Histologic Grade To Improve Prognosis
GSE6532 Definition of clinically distinct molecular subtypes in estrogen receptor positive breast carcinomas using genomic grade
Relations
Reanalyzed by GSE26971
Reanalyzed by GSE47561
Reanalyzed by GSE50564

Data table header descriptions
ID_REF probeset id
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 10.451001
1053_at 7.092225
117_at 7.51316
121_at 9.282128
1255_g_at 4.476062
1294_at 8.923702
1316_at 5.880662
1320_at 5.38414
1405_i_at 9.961853
1431_at 4.17679
1438_at 8.008741
1487_at 8.303607
1494_f_at 7.167491
1598_g_at 10.350939
160020_at 9.075497
1729_at 8.175763
1773_at 6.544269
177_at 6.064125
179_at 9.40281
1861_at 6.515098

Total number of rows: 22283

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
gsm65322.cel.gz 3.3 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap