|
| Status |
Public on Sep 15, 2022 |
| Title |
primary myoblasts, myogenic differentiation, DM2 |
| Sample type |
SRA |
| |
|
| Source name |
muscle
|
| Organism |
Anser cygnoides |
| Characteristics |
tissue: muscle
cell line: primary myoblasts
cell type: myoblasts
genotype: WT
treatment: myogenic differentiation
|
| Treatment protocol |
myogenic differentiation was induced by treatment of 2% horse serum, , and then cultured for 2 - 4 days to induce cells to differentiate into myotubes.
|
| Growth protocol |
primary myoblasts were maintained in DMEM supplemented with 15% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each goose tissue/cell using MagZol Reagent (Magen, Guangzhou, China)
RNA libraries for RNA-seq were prepared using TruSeq Stranded Total RNA HT Sample Prep Kit(Illumina RS-122-2203)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
remove low quailty reads and adapter (software:cutadapt, fastqc)
Mapping (software:hisat2)
Back spilcing identify (software:tophat_fusing)
transcript assembly (software:StringTie)
Difference analysis (software:edgeR)
Assembly: AnsCyg
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Sep 12, 2022 |
| Last update date |
Oct 06, 2022 |
| Contact name |
Shuai Zhang Shuai Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
South China Agricultural University
|
| Street address |
Wushan Street, Tianhe District
|
| City |
guangzhou |
| ZIP/Postal code |
510642 |
| Country |
China |
| |
|
| Platform ID |
GPL32723 |
| Series (1) |
| GSE213147 |
Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts |
|
| Relations |
| BioSample |
SAMN30805783 |
| SRA |
SRX17533278 |
