|
| Status |
Public on Jan 20, 2011 |
| Title |
Drosophila-sens-control-2 |
| Sample type |
RNA |
| |
|
| Source name |
Eye Discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Eye Discs
age: 3rd instar larvae
genotype: w; sensDF2RES/+; sensE1/+
|
| Growth protocol |
Crosses were reared at room temperature and wandering 3rd instar larvae were dissection to obtain eye imaginal discs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from 3rd instar eye discs (antennae removed)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and were normalized using the GCRMA algorithm implemented in the affy package using Bioconductor and R
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Aug 28, 2018 |
| Contact name |
S Lawrence Zipursky |
| Organization name |
Howard Hughes Medical Institute
|
| Department |
Biological Chemistry
|
| Lab |
5-784 MacDonald Research Laboratories
|
| Street address |
675 Charles E. Young Drive South
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE26717 |
Microarray analysis of R7 and R8 targeting |
|
| Relations |
| Reanalyzed by |
GSE119084 |
