|
| Status |
Public on Sep 01, 2012 |
| Title |
C2C12_miR-22 over-expression, replica 3 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cells, microRNA overexpression
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
protocol: miR-22 over-expression
|
| Treatment protocol |
microRNA mimics (Qiagen) were transfected using Hiperfect reagent (Qiagen) at 50nM concentration. After 36hrs total RNA was collected. The experiment was repeated three times
|
| Growth protocol |
C2C12 at p27 were grown in DMEM 10%FBS at 60-80% confluence
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
GeneChip WT Sense Target Labeling and Control Reagents, P/N 900652
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash, and Stain Kit, P/N 900720
|
| Scan protocol |
gene chip scanner 3000 7G
|
| Description |
miR-22-3
|
| Data processing |
The data were pre-processed with AGCC (Affymetrix) and Expression Console 1.1 using RMA
|
| |
|
| Submission date |
Jan 20, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Gabriele Bucci |
| E-mail(s) |
[email protected]
|
| Organization name |
Università vita e Salute Ospedale San Raffaele
|
| Department |
Center of Translational Genomics and Bioinformatics
|
| Lab |
GFU
|
| Street address |
Via Olgettina 60
|
| City |
Milan |
| ZIP/Postal code |
20132 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26764 |
Gene expression profiling of miR-regulated genes in proliferating C2C12 |
|
