|
| Status |
Public on Apr 18, 2012 |
| Title |
T cell Acute Lymphoblastic Leukemia 1 |
| Sample type |
RNA |
| |
|
| Source name |
The same T cell ALL as LALT obtained from the spleen of mouse
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: T cell ALL
|
| Growth protocol |
Samples 421, 739, 1 and 226 have been obtained from spleen of immunodeficient mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested with Ultraspec Reagent (Biotecx. Houston, TX, USA) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HuGene 1.0 ST chip according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
|
| Data processing |
RMA (R/Bioconductor processing)
|
| |
|
| Submission date |
Jan 21, 2011 |
| Last update date |
Apr 19, 2012 |
| Contact name |
Xabier Agirre |
| E-mail(s) |
[email protected]
|
| Phone |
+34 948194700
|
| Organization name |
CIMA
|
| Department |
Oncology
|
| Street address |
Pio XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE26789 |
The HDAC inhibitor panobinostat (LBH589) inhibits Acute Lymphoblastic leukemia (ALL) in vitro and in vivo in a new characterized human ALL mice model (ALL-B and ALL-T) |
| GSE26807 |
The HDAC inhibitor panobinostat (LBH589) inhibits Acute Lymphoblastic leukemia (ALL) in vitro and in vivo in a new characterized human ALL mice model |
|
