|
| Status |
Public on Dec 15, 2011 |
| Title |
HL60 Gleevec-treated_A |
| Sample type |
RNA |
| |
|
| Source name |
ATCC (CCL-240)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell line: HL60
disease: acute promyelocytic leukemia
genotype/variation: BCR-ABL deficient
agent: Gleevec
|
| Treatment protocol |
Gleevec-treated and non-treated cells.
|
| Growth protocol |
Cells were cultured in Roswell Park Memorial Institute medium (RPMI) supplemented with 10% heat inactivated fetal bovine serum (HIFBS) (Gibco, Invitrogen), 2mM L-glutamine (Gibco, Invitrogen) and 100U/ml penicillin and streptomycin (Gibco, Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen) with modifications. Cells were first washed with PBS twice and then lysed in TRIzol (Invitrogen) before purification using RNA extraction columns and buffers provided in the kit. The RNA concentration was quantified using Nanodrop (Thermo Scientific). 500ng total RNA was used for cRNA amplification using Illumina TotalPrep RNA Amplification kit (Ambion). Steps were carried out by following the instructional manual in the kit.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Scanner used: Illumina BeadArray Reader Scanning protocol: Scan factor of 1, PMT525 Software: Illumina Beadscan version 3.0
|
| Description |
1699538170_A
|
| Data processing |
Data is extracted using Illumina Genomestudio version 2010.2, Expression Module version 1.7.0 Data were analyzed using Partek GS6.4 Ver 6.09.0922. Normalization algorithm: RMA Analysis was performed using ANOVA.
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
Dec 15, 2011 |
| Contact name |
Sherry Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
National University of Singapore
|
| Department |
Biochemistry
|
| Street address |
21 Lower Kent Ridge Road
|
| City |
Singapore |
| ZIP/Postal code |
119077 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE26821 |
Regulation of hTERT by BCR-ABL at multiple levels in K562 cells |
|
