|
| Status |
Public on Dec 06, 2011 |
| Title |
rice leaf, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
rice matured leaf
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: leaf
|
| Growth protocol |
Rice immatured seed after 7-8 days pollination were harvested and the embryo was removed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 C on Affymetrix Rice Genome Array. GeneChips were washed and stained in the Affymetrix GCS3000.
|
| Scan protocol |
GeneChips were scanned using the Alpha Innotech AlphaScan.
|
| Description |
gene expression data from rice matured leaf
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
Dec 06, 2011 |
| Contact name |
Feng Tan |
| E-mail(s) |
[email protected]
|
| Phone |
662-325-3558
|
| Fax |
6623258664
|
| Organization name |
Mississippi State University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Peng's Lab
|
| Street address |
Dorman Hall 402
|
| City |
Mississippi State |
| State/province |
MS |
| ZIP/Postal code |
9762 |
| Country |
USA |
| |
|
| Platform ID |
GPL2025 |
| Series (2) |
| GSE26840 |
Express data from rice endosperm |
| GSE27064 |
Rice endosperm: mRNA profiling and H3K27me3 occupancy |
|
