|
| Status |
Public on Aug 03, 2011 |
| Title |
retina_2 months_lane_2 |
| Sample type |
SRA |
| |
|
| Source name |
neural retina
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J - 129SvEvTac
tissue: neural retina
age: 2 months
|
| Growth protocol |
Neural retina from five 2 month old C57BL/6J mice, mixed gender
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA-Seq using 5 ug total RNA; insert size 350bp +/- 25bp
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
96 million 120bp paired-end sequence reads were obtained from the 4 channels of an Illumina flow cell. The reads were aligned to the mouse transcriptome and genome (mm9) using the RNA-Seq Unified Mapper (RUM).
|
| |
|
| Submission date |
Jan 29, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric Pierce |
| E-mail(s) |
[email protected]
|
| URL |
http://www.masseyeandear.org/research/ophthalmology/laboratories/oculargenomics/
|
| Organization name |
Massachusetts Eye and Ear Infirmary
|
| Department |
Ophthalmology
|
| Lab |
Ocular Genomics Institute
|
| Street address |
243 Charles Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL11002 |
| Series (1) |
| GSE26248 |
Comparative Analysis of RNA-Seq Alignment Algorithms and the RNA-Seq Unified Mapper (RUM). |
|
| Relations |
| SRA |
SRX088979 |
| BioSample |
SAMN00691503 |
