|
| Status |
Public on Oct 15, 2022 |
| Title |
Frontal lobe |
| Sample type |
SRA |
| |
|
| Source name |
Hippocampus
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Hippocampus
sample type: Pooled RNA from 27 individuals
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA isolated by a modified guanidinium thiocyanate method (1) followed by poly A+ RNA selection with two rounds of oligo(dT)-cellulose columns (2).
direct cDNA sequencing (SQK-DCS109)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
PromethION |
| |
|
| Description |
Frontal_lobe_PAG06132
|
| Data processing |
guppy for basecalling
pychopper for trimming and orientation
minimap2 for mapping
stringtie to generate GFF annotation
Assembly: hg38 and GENCODE v38
Supplementary files format and content: TXT with gene expression matrix
|
| |
|
| Submission date |
Oct 13, 2022 |
| Last update date |
Oct 17, 2022 |
| Contact name |
Emil Karl Gustavsson |
| E-mail(s) |
[email protected]
|
| Organization name |
University College London
|
| Street address |
20c Guilford St
|
| City |
London |
| ZIP/Postal code |
WC1N 1DZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL26167 |
| Series (1) |
| GSE215459 |
Pseudogenes limit the identification of novel common transcripts generated by their parent genes |
|
| Relations |
| BioSample |
SAMN31275419 |
| SRA |
SRX17883191 |
