|
| Status |
Public on Oct 20, 2022 |
| Title |
TH_827 |
| Sample type |
RNA |
| |
|
| Source name |
Control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood
age: 40
gender: Female
disease: healthy control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNAs was isolated using the Qiagen miRNeasy Kit according to manufacturers recommendations.
|
| Label |
Cyanine 3-pcp
|
| Label protocol |
using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
| |
|
| Hybridization protocol |
The expression profile of 2549 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations
|
| Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
| Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized and log2 transformed.
quantil normalized, log2 transformed intensity values for all samples in set
|
| |
|
| Submission date |
Oct 17, 2022 |
| Last update date |
Oct 22, 2022 |
| Contact name |
Masood Abu-Halima |
| E-mail(s) |
[email protected]
|
| Organization name |
Saarland University
|
| Department |
Human genetics
|
| Street address |
kirrberger st
|
| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21576 |
| Series (2) |
| GSE215940 |
Expression profiling analysis reveals key microRNA–mRNA interactions in patients with transposition of the great arteries and systemic left and right ventricles [miRNA] |
| GSE215941 |
Expression profiling analysis reveals key microRNA-mRNA interactions in patients with transposition of the great arteries and systemic left and right ventricles |
|
