tissue: Papillary Thyroid Carcinoma morphology of papillary carcinomas: tall cell variant of papillary carcinoma ret/ptc translocation: N braf t1799a mutation: Y pax8/pparg translocation: N kras, nras, or hras mutation: N comment: no comment
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from the tissue lysate using Trizol (Invitrogen) and cleanup performed with an RNeasy kit (Qiagen)
Label
biotin
Label protocol
3-5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Data processing
Ann Arbor quantile-normalized trimmed-mean method, PMID: 15705192.