|
| Status |
Public on Jun 30, 2012 |
| Title |
C.elegans_8days_GC1-2 |
| Sample type |
RNA |
| |
|
| Source name |
Ground control
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2 wild type
developmental stage: adult
|
| Growth protocol |
Approximately 50 L1 larvae were grown in 2 mL M9. Worms were cultured in 12 mL S basal medium contained bacterial feeds for 8days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Worms were homogenized with four rounds of 20-second beating at 5000 rpm in a Micro Smash TM MS-100 bead beater with 3 minutes of cooling on ice between rounds. Total RNA was extracted using an Isogen RNA purification kit following the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was amplified using the Low Input Quick Amp Labeling Kit protocol. Cy3 labeled cRNA was produced according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Labeled cRNA (1.65 µg/sample) was hybridized to a C. elegans oligo microarray using a gene expression hybridization kit at 65 °C for 17 hours.
|
| Scan protocol |
Array were scanned with an Agilent Scanner and processed with Agilent Feature Extraction Software.
|
| Data processing |
Microarray data were analyzed using Gene Spring software (Agilent Technologies). Normalized data were produced by "Normalize to 75 percentile sift protocol’’ and "baseline to median of control sample protocol’’. Arrays were filtered on expression (20-100)th percentile in raw data.
|
| |
|
| Submission date |
Feb 16, 2011 |
| Last update date |
Jun 30, 2012 |
| Contact name |
Akira Higashibata |
| E-mail(s) |
[email protected]
|
| Phone |
050-3362-3898
|
| Organization name |
Japan Aerospace Exploration Agency
|
| Department |
Department of Space Biology and Microgravity Sciences
|
| Lab |
ISS Science Project office
|
| Street address |
2-1-1,Sengen
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8505 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11346 |
| Series (1) |
| GSE27338 |
Microgravity effect on C. elegans N2/VC (CERISE, 8days) |
|
