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Status |
Public on Feb 18, 2011 |
Title |
db/+ mouse sample 103 |
Sample type |
RNA |
|
|
Source name |
24 week db/+ mouse sciatic nerve
|
Organism |
Mus musculus |
Characteristics |
tissue: sciatic nerve strain: BKS genotype/variation: db/+ age: 24 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from sciatic nerve using a commercially available silica gel-based isolation protocol (RNeasy Mini Kit, QIAGEN Inc., Valencia, CA) including an on-column DNase digestion following the manufacturer's protocol. RNA quantity and quality was measured by microfluid electrophoresis using the RNA 6000 Pico LabChip® on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
Label |
biotin
|
Label protocol |
Of the total RNA, 75 ng from each sample was amplified and biotin-labeled using the Ovation™ Biotin-RNA Amplification and Labeling System (NuGEN Technologies Inc., San Carlos, CA) according to the manufacturer’s protocol
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|
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Hybridization protocol |
Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
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Description |
Gene expression data from 24 week db/+ mouse sciatic nerve
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Data processing |
The Affymetrix CEL files were initially analyzed using a local version of the GenePattern genomic analysis platform from the Broad Institute. The samples were Robust Multi-array Average (RMA).
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|
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Submission date |
Feb 17, 2011 |
Last update date |
Jan 06, 2012 |
Contact name |
Manjusha Pande |
E-mail(s) |
[email protected]
|
Organization name |
University of Michigan
|
Department |
BRCF
|
Lab |
Bioinformatics Core
|
Street address |
2800 Plymouth Rd.
|
City |
Ann Arobr |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL9746 |
Series (2) |
GSE27382 |
Transcriptional Profiling of Diabetic Neuropathy in BKS-db/db Mouse, a Model for Type 2 Diabetes |
GSE34889 |
Molecular Mechanisms of Diabetic Neuropathy: A Transcriptional Profling Study of db/db Mouse Sciatic Nerve |
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