|
| Status |
Public on Jan 27, 2012 |
| Title |
1 WT 500T |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from Mouse Embryonic Fibroblasts NFAT5 wild-type 5.4
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129sv/ev
genotype/variation: NFAT5 wild-type 5.4
cell type: MEFs
treatment: hypertonic with 1 h pretreatment using 100 nM Torin1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Kit QIAGEN
|
| Label |
Biotin
|
| Label protocol |
100-300 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Mouse Gene 1.0 ST Array (Affymetrix) using a GeneChip Hybridization Oven 640
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression data from Mouse Embryonic Fibroblasts
|
| Data processing |
The data were analyzed using the RMA algorithm and then SAM was applied to calculate significant differential expression between samples using R 2.11.1 and GEOsubmission 1.0.
|
| |
|
| Submission date |
Feb 24, 2011 |
| Last update date |
Jan 27, 2012 |
| Contact name |
Jose Aramburu |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Universitat Pompeu Fabra
|
| Department |
Experimental and Health Sciences
|
| Lab |
Immunology
|
| Street address |
Carrer Doctor Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27485 |
Gene expression from Mouse Embryonic Fibroblasts |
|
