|
| Status |
Public on Aug 06, 2011 |
| Title |
GM06990_Exp |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Foreskin fibroblast
|
| Growth protocol |
The GM06990 cells were cultured as recommended by the Coriell repository. The fibroblast cells were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% Fetal Bovine Serum, 2 mM Glutamine and Penicillin-Streptomycin (Invitrogen) in a 37°C incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The mRNA was converted to dsDNA using SuperScript Double-Strand cDNA Synthesis kit (Invitrogen) with T7-Oligo(dT)24 (5’- GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGTTTTTTTTTTTTTTTTTTTTTTTT -3’). DNA labeling and hybridization to the microarrays were performed using a published technique (Selzer, Richmond, Pofahl, Green, Eis, Nair, Brothman and Stallings 2005).
|
| Label |
Cy5
|
| Label protocol |
see manufacturer's web site www.nimblegen.com
|
| |
|
| Hybridization protocol |
see manufacturer's web site www.nimblegen.com
|
| Scan protocol |
see manufacturer's web site www.nimblegen.com
|
| Description |
GPL11219
|
| Data processing |
The raw data (.pair file) was analyzed the NimbleScan software package (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Feb 24, 2011 |
| Last update date |
Aug 06, 2011 |
| Contact name |
John Greally |
| E-mail(s) |
[email protected]
|
| Phone |
7186781234
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Genetics
|
| Street address |
1301 Morris Park Avenue
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL11219 |
| Series (2) |
| GSE27518 |
LATE-REPLICATING HETEROCHROMATIN IS CHARACTERISED BY DECREASED CYTOSINE METHYLATION IN THE HUMAN GENOME (expression) |
| GSE27538 |
LATE-REPLICATING HETEROCHROMATIN IS CHARACTERISED BY DECREASED CYTOSINE METHYLATION IN THE HUMAN GENOME |
|
