|
| Status |
Public on May 01, 2011 |
| Title |
ES6 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129S6
cell type: embryonic stem cells
|
| Treatment protocol |
For samples 4, 5 and 6, cells were trypsinized and cells harvested by centrifugation. For the rest of the samples, feeders were eliminated before cells were harvested.
|
| Growth protocol |
Samples 1, 2, 3, 7, 8 and 9 were maintained in mouse ES media. Samples 4, 5 and 6 were maintain in fibroblast media. All samples were incubated at 37 degress Celsius in a humidified chamber containg 5% carbondioxide.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used following manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Ambion WT Expression Kit was used following manufacturer's instruction
|
| |
|
| Hybridization protocol |
GeneChip WT Terminal Labeling and Hybridization kit from Affymetrix following manufacturer's instructions.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from R1 ES cells
|
| Data processing |
Data was analyzed using Partek Genomics Suite v6.5.
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
May 01, 2011 |
| Contact name |
Frederick Anokye-Danso |
| Organization name |
University of Pennsylvania
|
| Department |
Medicine
|
| Lab |
Cardiology
|
| Street address |
421 Curie Blvd Room 938
|
| City |
Philadelphia |
| State/province |
Pa |
| ZIP/Postal code |
190104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27814 |
Expression data of reprogrammed miR-302/367-iPS cells |
|
