|
| Status |
Public on Feb 01, 2012 |
| Title |
Embryo at 12DAF, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Rice embryo at 12 DAF
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: Embryo
developmental stage: 12 day after flowering
|
| Treatment protocol |
All the meterials were collected quickly, and frozen in liquid nitrogen.
|
| Growth protocol |
Rice (Oryza sativa, japonica cultivar Zhonghua 11), was cultivated at day/night temperatures (28/22 °C) in phytotron.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted using TRIzol reagent (Invitrogen) accordding to manufactures manual
|
| Label |
biotin
|
| Label protocol |
RNA was labeled for hybrid with the Affymetrix GeneChip Rice Genome array according to the manufactures protocol. (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix® GeneChip® Operating Software Version 1.0 was used for washing, staining, and scanning as described in the supplier’s protocol.
|
| Scan protocol |
Scanning procedures of the arrays were carried out using Affymetrix GeneChip Scanner 3000 as described in the Affymetrix technical manual.
|
| Description |
Embryo were collected at 12 day after flowering
|
| Data processing |
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 09, 2011 |
| Last update date |
Feb 01, 2012 |
| Contact name |
Hong-Wei Xue |
| E-mail(s) |
[email protected]
|
| URL |
http://www.plantsignal.cn
|
| Organization name |
Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences
|
| Street address |
300 Fenglin Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE27856 |
Expression data from rice embryo and endosperm development |
|
