|
| Status |
Public on Feb 28, 2024 |
| Title |
WT_XA_4h_2 |
| Sample type |
SRA |
| |
|
| Source name |
WT_XA_4h
|
| Organism |
Thermothelomyces thermophilus |
| Characteristics |
strain: ATCC 42464
cell type: Mycelium
|
| Growth protocol |
Precultured mycelium from M. thermophila WT strain (ATCC42464) were shifted into 100 mL 1×Vogel’s salt with 2% Xylan or 2% AX (1% Avicel and 1% Xylan) in 250-mL Erlenmeyer flask for the induciton of additional 4 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mycelia were harvested by vacuum filtration with Whatman filter paper, frozen immediately in liquid nitrogen. Total RNA from frozen samples was isolated using TRIzol reagent and an additional clean-up was performed for DNA degradation using the RNeasy mini kit (Qiagen)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence with NGSQCToolkit (v2.3).
Clear reads were mapped to Myceliophthora thermophila ATCC 42464 genome using Tophat (version 2.0.8b) with parameters -i 10 -g 1 --segment-length 24 --b2-very-sensitive --no-convert-bam --library-type fr-unstranded.
The counts of reads uniquely mapped to only one gene for each gene were calculated by HTseq-count(version 0.13.5).
Assembly: Myceliophthora thermophila ATCC 42464(https://genome.jgi.doe.gov/Spoth2/Spoth2.home.html)
Supplementary files format and content: tab-delimited text files including Reads count for each sample.
|
| |
|
| Submission date |
Jan 07, 2023 |
| Last update date |
Feb 28, 2024 |
| Contact name |
Jingen Li |
| E-mail(s) |
[email protected]
|
| Phone |
+86-02284861948
|
| Organization name |
Tianjin institute of industrial biotechnology,Chinses Academy of Sciences
|
| Street address |
Xiqi Dao 32, Tianjin Airport Economic Area
|
| City |
Tianjin |
| ZIP/Postal code |
300308 |
| Country |
China |
| |
|
| Platform ID |
GPL27455 |
| Series (1) |
| GSE222371 |
Orthogonal metabolism of cellodextrin and xylodextrin is the keystone of synchronous utilization of cellulose and hemicellulose in Myceliophthora thermophila |
|
| Relations |
| BioSample |
SAMN32628927 |
| SRA |
SRX18965490 |
