|
| Status |
Public on Oct 19, 2011 |
| Title |
testis, Repro9 mutant, 17dpp, Rep4 |
| Sample type |
RNA |
| |
|
| Source name |
mutant testis at 17dpp
|
| Organism |
Mus musculus |
| Characteristics |
tissue: testis
genotype: repro9/repro9
age: 17dpp
|
| Growth protocol |
Regular mouse growth conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed from homogenized testes using Qiagen RNAeasy minikit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
According to Affymetrix standard procedure, 10 micrograms of fragmented cRNA were hybridized to an array for 16-18 hours at 45°C with constant rotation at 60 rpm. After hybridization, the array was washed and stained using an Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using theAffymetrix GeneArray3000
|
| Description |
mutant testis at 17dpp
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 17, 2011 |
| Last update date |
Oct 19, 2011 |
| Contact name |
John Schimenti |
| E-mail(s) |
[email protected]
|
| URL |
http://web.me.com/tcomplex/
|
| Organization name |
Cornell University
|
| Department |
Biomedical Sciences
|
| Lab |
Schimenti
|
| Street address |
T9014A
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28025 |
Comparative gene expression analysis of repro9/repro9 and wild type testes from 14 and 17 day mice |
|
